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The method of claim 1, wherein the method produces an enantiomerically pure or enantiomerically enriched 1,2-amino alcohol, comprising the steps of: The method of claim 2, wherein the alcohol oxidase is alditol oxidase or its mutants, or the alcohol dehydrogenase is selected from the group consisting of AlkJ from Pseudomonas putida, AlkJ homologue from Sphingomonas sp.
HXN , dihydrodiol dehydrogenase, and mutants thereof. The method of claim 2, wherein the alkene has the formula I: R1 to R3 independently represent H, an alkyl group, an alkenyl group, an alkynyl group, a cycloalkyl group, a cycloalkenyl group, an aryl group, an aryl alkyl group, a heterocyclic group, and a heterocyclic alkyl group, which groups are substituted or unsubstituted by one or more substituents, provided that at least one of R1 to R3 is not H. The method of claim 2, wherein the method comprises the use of one or more reactive components selected from the group consisting of cells, immobilized cells, cell extract, isolated enzymes and immobilized enzymes.
The method of claim 8, wherein the alcohol oxidase is alditol oxidase or its mutants, or the alcohol dehydrogenase is selected from the group consisting of AlkJ from Pseudomonas putida, AlkJ homologue from Sphingomonas sp.
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However, our insight into the organization of these different enzymatic activities into a bioactive tertiary structure remains rudimentary. Fragments of Mononegavirales polymerases analyzed to date cannot restore bioactivity through trans-complementation, unlike the related L proteins of segmented NSVs. Through a comprehensive in silico and experimental analysis of domain intersections, we defined MeV L position as an interdomain candidate in addition to the previously reported residue Splitting of MeV L at residue created fragments that were unable to physically interact and trans-complement, but strikingly, these activities were reconstituted by the addition of dimerization tags to the fragments.
Equivalently split fragments of NiV, RSV, and MeV L oligomerized with comparable efficiency in all homo- and heterotypic combinations, but only the homotypic pairs were able to trans-complement.
Feb 24, · All enzymatic activities required for genomic replication and transcription of nonsegmented negative strand RNA viruses (or Mononegavirales) are believed to be concentrated in the viral polymerase (L) protein.
Source-to-sink sediment transport from the river mouth to the continental shelf and then to the abyssal basin is investigated at two time scales: The results firstly show the high diversity of clay mineralogical and geochemical compositions in riverbed surface sediments surrounding the South China Sea, e. The formation of these fluvial sediments through chemical weathering in surrounding drainage systems is controlled principally by the East Asian monsoon climate with warm temperature and high precipitation, and subordinately by tectonic activity and specific lithological character.
The basin-wide distribution of clay mineral assemblages combined with neodymium and strontium isotopic compositions reflects strong provenance control and differential settling effects. The differential settling of kaolinite in slightly saline and proximal regions relative to smectite in more saline and distal regions is well demonstrated in the South China Sea. Through combining clay mineralogical distributions on the seafloor with observed oceanic current systems, the modern transport pathways can be well established, e.
High-resolution sediment dynamic analysis for the Late Quaternary reveals different sediment transport patterns in the northern, western, and southern South China Sea when the land—sea configuration dramatically changed during glacial conditions. In the north, the terrigenous dispersal is mainly controlled by provenance supply and oceanic current transport. In the west, the clay mineral assemblage reflects variations of the prevailing surface current influenced by monsoon winds.
Warneford was awarded a Victoria Cross for his actions. Destroyed by British bombing in its hangar at Evere on 7 June On 17 December , captained by Dr. All rear gas cells were punctured and the front engine car was hit and later fell off.
Jul 13, · Thursday, July 13, ; LZ Arts and Crafts. BOOK ‘N STITCHERS, all ages, p.m. third Tuesdays, Bealeton Library, Willow Dr. Bring .
The attached Notice of Annual Meeting of Shareholders and Proxy Statement contain details of the business to be conducted. This year, we have further enhanced the clarity of the information we provide to you. This proxy statement demonstrates our continuing commitment to move beyond required disclosures to simplify and more clearly explain the information you need. Throughout the year, we conduct considerable outreach to shareowners on a variety of topics, and we are guided by your feedback.
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Built at the Zeppelin Company works Luftschiffbau Zeppelin in Friedrichshafen am Bodensee Lake Constance , Germany, between and , the LZ had a design patterned on that of the LZ , which the company had delivered as a war reparation to the U. Hugo Eckener promptly began a two-year campaign of lobbying the German Government for funds and permission to proceed with construction of a new airship for Germany.
Using gasoline alone it was able to cruise for 67 hours, and up to hours using both.
See more EBM Papst LZ22 Guard Finger x51mm Email to friends Share on Facebook – opens in a new window or tab Share on Twitter – opens in a new window or tab Share on Pinterest – .
Published online Jan 3. This article has been cited by other articles in PMC. Abstract All enzymatic activities required for genomic replication and transcription of nonsegmented negative strand RNA viruses or Mononegavirales are believed to be concentrated in the viral polymerase L protein. However, our insight into the organization of these different enzymatic activities into a bioactive tertiary structure remains rudimentary. Fragments of Mononegavirales polymerases analyzed to date cannot restore bioactivity through trans-complementation, unlike the related L proteins of segmented NSVs.
Through a comprehensive in silico and experimental analysis of domain intersections, we defined MeV L position as an interdomain candidate in addition to the previously reported residue Splitting of MeV L at residue created fragments that were unable to physically interact and trans-complement, but strikingly, these activities were reconstituted by the addition of dimerization tags to the fragments. Equivalently split fragments of NiV, RSV, and MeV L oligomerized with comparable efficiency in all homo- and heterotypic combinations, but only the homotypic pairs were able to trans-complement.
These results demonstrate that synthesis as a single polypeptide is not required for the Mononegavirales polymerases to adopt a proper tertiary conformation.
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External links 8 Principal characteristics The pink ovals depict hydrogen cells inside the LZ , the magenta elements are Blaugas cells. The full-resolution picture labels more internals. The principal feature of Zeppelin’s design was a fabric-covered rigid metal framework made up from transverse rings and longitudinal girders containing a number of individual gasbags.
The framework of most Zeppelins was made of duralumin. Early Zeppelins used rubberised cotton for the gasbags, but most later craft used goldbeater’s skin , made from the intestines of cattle. The first Zeppelins had long cylindrical hulls with tapered ends and complex multi-plane fins. They were propelled by several engines , mounted in gondolas or engine cars, which were attached to the outside of the structural framework.
Some of these could provide reverse thrust for manoeuvring while mooring. A comparatively small compartment for passengers and crew was built into the bottom of the frame, but in later Zeppelins this was not the entire habitable space; they often carried passengers or cargo internally for aerodynamic reasons. History Early designs Ferdinand von Zeppelin Count Ferdinand von Zeppelin ‘s serious interest in airship development began in , when he took inspiration from a lecture given by Heinrich von Stephan on the subject of “World Postal Services and Air Travel” to outline the basic principle of his later craft in a diary entry dated 25 March Convinced of the potential importance of aviation, he started working on various designs in , and had completed detailed designs by An official committee reviewed his plans in , and he received a patent, granted on 31 August , with Theodor Kober producing the technical drawings.
Manufactured in The Netherlands. The sequence exhibited a high degree of relatedness to both Sendai virus, a PIV1 virus recovered from mice, and human PIV3 HPIV3 with regard to cis-acting regulatory regions and protein-coding sequences. The replication of rHPIV1 in vitro and in the respiratory tract of hamsters was similar to that of its biologically derived parent virus.
The human PIVs have a similar genomic organiza- tion, although significant differences occur in the Pgene [1,2].
Eat Fresh, Eat Well, Eat Local Better!! La Scala Italian Restaurant,voted the area”s Best Italian Restaurant, is located in historic downtown Lafayette and.
Mary moved into a tie with St. Basil for the lead yes- , Today we have illustrated three ways to turn impossible situations into potential pars. These are shots, suggested by my friend, trick shot artist Paul Hahn, that any golfer can play. Mary picked up a pair of runs in the fourth inning when Jack Fredland singled in Bob Kine and Rich Stam- record after blanking rival coach at Reynoldsville High Duquesne, , yesterday, orosKy. Goettler singled in one run and in the fourth and added a homer in the seventh for the St.
He succeeds Bob Holmes, who is going to Juniata College as an assistant. Each pitched two innings in the shutout over the Dukes. Rider plays Princeton in the first game and the two winners clash Saturday afternoon for the right to represent District 2 in the College World Series starting June 7 0 was from the eastern part of team in the 87th annual IC4A track and field championships tomorrow and Saturday at today.
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Design and development The Hindenburg under construction The Hindenburg had a duralumin structure, incorporating 15 Ferris wheel -like bulkheads along its length, with 16 cotton gas bags fitted between them. The bulkheads were braced to each other by longitudinal girders placed around their circumferences. The airship’s outer skin was of cotton doped with a mixture of reflective materials intended to protect the gas bags within from radiation , both ultraviolet which would damage them and infrared which might cause them to overheat.
The gas cells were made by a new method pioneered by Goodyear using multiple layers of gelatinized latex rather than the previous goldbeater’s skins. A stylized world map covered the wall of the lounge. Long slanted windows ran the length of both decks.
Explore the George Eastman Museum’s collections online. More than a quarter of a million objects from the photography, technology, and George Eastman Legacy collections are now searchable, and more of the museum’s vast holdings will be added on an ongoing basis.
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It was introduced as a model and continued until when the current Hardbody style model D was introduced as a The features an independent front suspension with torsion bar springs, a leaf spring live axle rear suspension and a 4 cylinder engine displacing 2. Longbed and extra-cab models were also available. Nissans have a reputation for having aftermarket parts that are expensive and hard to find.
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Return policy details Returns accepted Most purchases from business sellers are protected by the Consumer Contract Regulations which give you the right to cancel the purchase within 14 days after the day you receive the item. Find out more about your rights as a buyer – opens in a new window or tab and exceptions – opens in a new window or tab. Seller assumes all responsibility for this listing. Postage and packaging The seller hasn’t specified a postage method to Russian Federation.